Download Biophysics of RNA-Protein Interactions PDF

Biophysics of RNA-Protein Interactions

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Publisher : Springer Nature
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ISBN : 1493997262
Pages : 249 pages
Rating : 4.9/5 (997 Download)

Download Free Biophysics of RNA-Protein Interactions PDF by Chirlmin Joo Full Book and published by Springer Nature. This book was released on 2019-09-19 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.


Download Biophysics of DNA-Protein Interactions PDF

Biophysics of DNA-Protein Interactions

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Publisher : Springer Science & Business Media
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ISBN : 0387928081
Pages : 350 pages
Rating : 4.9/5 (928 Download)

Download Free Biophysics of DNA-Protein Interactions PDF by Mark C. Williams Full Book and published by Springer Science & Business Media. This book was released on 2010-10-05 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.


Download Protein-binding and RNA-binding Properties of RNA-binding Proteins FBF and RBM39 PDF

Protein-binding and RNA-binding Properties of RNA-binding Proteins FBF and RBM39

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ISBN : 9781303073212
Pages : 432 pages
Rating : 4.2/5 (218 Download)

Download Free Protein-binding and RNA-binding Properties of RNA-binding Proteins FBF and RBM39 PDF by Joann Wu Full Book and published by . This book was released on 2012 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-transcriptional regulation of gene expression is critical in the development, physiology, and reproduction of multicellular organisms. Large dynamic multi-protein complexes formed through extensive protein*protein and protein*RNA interactions control mRNA processing, splicing, nuclear transport, localization, stability, and translation. However, despite the biological importance of these regulatory complexes, there is sparse molecular information describing the interactions involved in their assembly. In these studies, two categories of RNA-binding proteins that act as foundations for these complexes are examined: the Pu milio and F[barbelow]BF (PUF) proteins and R[barbelow]NA r[barbelow]ecognition m[barbelow]otif (RRM) domain-containing proteins. While there is a wealth of knowledge for PUF protein interactions with RNA, there is limited information describing the equally essential interactions between PUF proteins and their protein binding partners. Defined in these studies are the molecular bases for the developmentally essential interactions between Caenorhabditis elegans PUF protein f[barbelow]em-3 b[barbelow]inding f[barbelow]actor (FBF) and the two protein binding partners: g[barbelow]erml[barbelow]ine d[barbelow]evelopment defective-3[barbelow] (GLD-3) and c[barbelow]ytoplasmic p[barbelow]olyadenylation element b[barbelow]inding-1 (CPB-1). Short specific sequences in unstructured regions of the GLD-3 and CPB-1 proteins are critical for interaction with FBF, while a loop connecting PUF repeats R7 and R8 in FBF is essential for association with both GLD-3 and CPB-1. The PUF protein*binding partner complex FBF*GLD-3 is capable of forming a stable complex on RNA FBF regulatory elements in the 3' u[barbelow]nt[barbelow]ranslated r[barbelow]egions (UTR) of specific germline developmental mRNAs, and may control their expression through several proposed mechanisms. Although the RRM domains that characterize RRM-containing proteins adopt the same vary greatly in: amino acid sequence; DNA, RNA, and protein-binding abilities; and mode of interaction with these biological molecules. These studies potentially identify the first member of a unique class of RRMs that bind to RNA in a manner similar to RRMs that interact with proteins. These studies characterizing the protein- and RNA-binding properties of these RNA-binding proteins provide insight into the biologically essential molecular complexes involved in post-transcriptional gene regulation.


Download An Analysis of the Molecular Basis of TRNA-protein Interactions PDF

An Analysis of the Molecular Basis of TRNA-protein Interactions

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Publisher :
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ISBN :
Pages : 400 pages
Rating : 4.7/5 (789 Download)

Download Free An Analysis of the Molecular Basis of TRNA-protein Interactions PDF by Joseph John Rosa Full Book and published by . This book was released on 1976 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Biophysical approaches to translational control of gene expression PDF

Biophysical approaches to translational control of gene expression

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Publisher : Springer Science & Business Media
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ISBN : 1461439914
Pages : 320 pages
Rating : 4.4/5 (439 Download)

Download Free Biophysical approaches to translational control of gene expression PDF by Jonathan D. Dinman Full Book and published by Springer Science & Business Media. This book was released on 2012-09-20 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a premier resource on understanding the ribosome's essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis. As one of the central foundations in our understanding of the biology at the molecular level, this topic appeals to a wide audience, from bench researcher to clinician. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm.


Download Structures of Large RNA Molecules and Their Complexes PDF

Structures of Large RNA Molecules and Their Complexes

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Publisher : Academic Press
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ISBN : 0128019360
Pages : 654 pages
Rating : 4.0/5 (19 Download)

Download Free Structures of Large RNA Molecules and Their Complexes PDF by Full Book and published by Academic Press. This book was released on 2015-06-06 with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs. Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes


Download Introduction to Biomolecular Structure and Biophysics PDF

Introduction to Biomolecular Structure and Biophysics

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Publisher : Springer
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ISBN : 9811049688
Pages : 274 pages
Rating : 4.0/5 (49 Download)

Download Free Introduction to Biomolecular Structure and Biophysics PDF by Gauri Misra Full Book and published by Springer. This book was released on 2017-09-07 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive book presents a modern concept in biophysics based on recently published research. It highlights various aspects of the biophysical fundamentals and techniques that are currently used to study different physical properties of biomolecules, and relates the biological phenomenon with the underlying physical concepts. The content is divided into nine chapters summarizing the structural details of proteins, including recently discovered novel folds, higher order structures of nucleic acids, as well as lipids and the physical forces governing the macromolecular interactions which are essential for the various biological processes. It also provides insights into the recent advances in biophysical techniques including Hydrogen Deuterium Exchange with Mass Spectrometry (HDX-MS), Small angle X-ray scattering (SAXS) and Cryo Electron Microscopy (cryo EM), supplemented with interesting experimental data. It is a valuable reference resource for anyone with a desire to gain a better understanding of the fundamentals of biophysical concepts and techniques of important biomolecules.


Download RNA-Protein Interaction Protocols[ PDF

RNA-Protein Interaction Protocols[

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Publisher : Springer Science & Business Media
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ISBN : 1592596762
Pages : 481 pages
Rating : 4.5/5 (596 Download)

Download Free RNA-Protein Interaction Protocols[ PDF by Susan R. Haynes Full Book and published by Springer Science & Business Media. This book was released on 1999 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems. Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field.


Download Biophysics of RNA Folding PDF

Biophysics of RNA Folding

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Publisher : Springer Science & Business Media
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ISBN : 1461449545
Pages : 238 pages
Rating : 4.4/5 (449 Download)

Download Free Biophysics of RNA Folding PDF by Rick Russell Full Book and published by Springer Science & Business Media. This book was released on 2012-12-09 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.


Download Water and Biological Macromolecules PDF

Water and Biological Macromolecules

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Publisher : Palgrave
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ISBN : 9781349123612
Pages : 486 pages
Rating : 4.6/5 (617 Download)

Download Free Water and Biological Macromolecules PDF by Eric Westhof Full Book and published by Palgrave. This book was released on 2013-12-31 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Water and Biological Macromolecules presents an excellent description of the structural aspects of water molecules around biological macromolecules. Topics discussed include the properties of water in solid and liquid states; proteins, nucleic acids, polysaccharides, and lipids; and theoretical approaches for understanding the macroscopic observations and integrating microscopic descriptions. The nature and roles of hydration forces in macromolecular complexation and cell-cell interactions are explained, in addition to phenomena such as entropy-enthalpy compensation and the thermodynamic treatment of water bridging. Water and Biological Macromolecules will be a valuable reference for biophysicists, biochemists, and macromolecular biologists.


Download Issues in Biochemistry and Biophysics Research: 2013 Edition PDF

Issues in Biochemistry and Biophysics Research: 2013 Edition

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Publisher : ScholarlyEditions
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ISBN : 1490108653
Pages : 1218 pages
Rating : 4.1/5 (18 Download)

Download Free Issues in Biochemistry and Biophysics Research: 2013 Edition PDF by Full Book and published by ScholarlyEditions. This book was released on 2013-05-01 with total page 1218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Biochemistry and Biophysics Research: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Amino Acids. The editors have built Issues in Biochemistry and Biophysics Research: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Amino Acids in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biochemistry and Biophysics Research: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.


Download Biophysics PDF

Biophysics

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Publisher : CRC Press
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ISBN : 1498702465
Pages : 390 pages
Rating : 4.7/5 (72 Download)

Download Free Biophysics PDF by Mark C. Leake Full Book and published by CRC Press. This book was released on 2016-09-15 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: An Up-to-Date Toolbox for Probing Biology Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.


Download Biophysical Approaches to Translational Control of Gene Expression PDF

Biophysical Approaches to Translational Control of Gene Expression

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Publisher : Springer Science & Business Media
Release Date :
ISBN : 1461439906
Pages : 320 pages
Rating : 4.4/5 (439 Download)

Download Free Biophysical Approaches to Translational Control of Gene Expression PDF by Jonathan D. Dinman Full Book and published by Springer Science & Business Media. This book was released on 2012-07-27 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights major advances in researching a cell’s molecular machinery through analytical, computational, and imaging methods. It focuses on developing biophysical approaches to studying control of gene expression at the translational level.


Download Issues in Biochemistry and Biophysics Research: 2011 Edition PDF

Issues in Biochemistry and Biophysics Research: 2011 Edition

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Publisher : ScholarlyEditions
Release Date :
ISBN : 1464964017
Pages : 1905 pages
Rating : 4.9/5 (964 Download)

Download Free Issues in Biochemistry and Biophysics Research: 2011 Edition PDF by Full Book and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 1905 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Biochemistry and Biophysics Research: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Biochemistry and Biophysics Research. The editors have built Issues in Biochemistry and Biophysics Research: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biochemistry and Biophysics Research in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biochemistry and Biophysics Research: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.


Download Molecular Biophysics for the Life Sciences PDF

Molecular Biophysics for the Life Sciences

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Publisher : Springer Science & Business Media
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ISBN : 1461485487
Pages : 397 pages
Rating : 4.4/5 (485 Download)

Download Free Molecular Biophysics for the Life Sciences PDF by Norma Allewell Full Book and published by Springer Science & Business Media. This book was released on 2013-09-28 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate how the combination of theory and experiment leads to new insights and new questions.


Download Calorimetry PDF

Calorimetry

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Publisher : Academic Press
Release Date :
ISBN : 0128029072
Pages : 530 pages
Rating : 4.0/5 (29 Download)

Download Free Calorimetry PDF by Full Book and published by Academic Press. This book was released on 2016-01-12 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calorimetry, the latest volume in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Calorimetry is a highly technical experiment and it is easy for new practioners to get fooled into interpreting artifacts as real experimental results. This volume will guide readers to get the most out of their precious biological samples and includes topics on specific protocols for the types of studies being conducted as well as tips to improve the data collection. Most importantly, the chapters will also help to identify pitfalls that need to be avoided to ensure that the highest quality results are obtained. Contains timely contributions from recognized experts in this rapidly changing field Provides specific protocols and tips to improve data collection and ensure the highest quality results are obtained Covers research methods in calorimetry, and includes sections on topics such as differential scanning calorimetry of membrane and soluble proteins in detergents.


Download High Pressure Effects in Biophysics and Enzymology PDF

High Pressure Effects in Biophysics and Enzymology

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Publisher : Oxford University Press
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ISBN : 9780195344622
Pages : 400 pages
Rating : 4.6/5 (626 Download)

Download Free High Pressure Effects in Biophysics and Enzymology PDF by John L. Markley Full Book and published by Oxford University Press. This book was released on 1996-09-12 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Pressure Effects in Molecular Biophysics and Enzymology is designed to acquaint biochemists, biophysicists, and graduate students with advances in the application of high pressure in connection with spectroscopy as a research tool in the study of biomolecules. The 23 chapters written by leading authorities present an overview of current approaches to the use of high pressure in research on enzyme kinetics, protein folding and structure, lipid bilayer structure and organization, lipid-protein interaction, and DNA structure. This important, timely volume is the first devoted exclusively to high-pressure effects in biochemistry and will be the definitive reference in its subject for the next several years.